The purpose was to study the effect of anti-G straining maneuvers on the G-protective properties afforded by the anti-G ensemble used in the JAS 39 Gripen aircraft (AGE-39). Methods. Ten subjects were studied in a centrifuge using: (1) the AGE-39 in combination with full anti-G straining maneuvers (AGSM) throughout each high-G exposure (Full Maneuver; FM) and (2) the AGE-39 in combination with AGSM during the initial part of each high-G exposure (Reduced Maneuver; RM). G-intensity tolerance was established during exposures to Rapid Onset Rate (ROR) profiles with G-plataeu levels ranging from 6.5 to 9.0 G. G-endurance was studied during Simulated Aerial Combat Maneuvers (SACM) consisting of ten cycles of 5.5 to 7.5 G. Results. G-intensity tolerance was 8.8 G (=mean; range: 8.5-9.0 G) in the RM-condition and 9.0 G in all subjects in the FM-condition. Mean arterial pressure at eye level was 30-50 mmHg higher (p<0.001) in the FM than in the RM condition at any given ROR G-load. In the RM condition all subjects completed the SACM profile; the rating of perceived exertion (RPE) after the profile was 5.5 (3.5-7.0) on a 10-graded scale. In the FM condition 1 subject discontinued the SACM-profile prematurely because of fatigue, RPE was 7.0 (3.0-9.0) upon termination of the profile. Conclusions. The AGE-39 provides an efficient G-protection as illustrated by the finding that all subjects could tolerate 8.5 G while performing AGSM merely during the initial part of the high-G exposures. However, at 9.0 G, 40% of the subjects had to perform AGSM throughout the duration of the G-exposure. That arterial pressure was only 30-50 mmHg higher in the FM than RM condition may suggest that in the presence of muscular straining maneuvers the share to the arterial-pressure response contributed by pressure breathing is minute.