The therapeutic potential of N,N´bis-(2-mercaptoethyl)isophthalamide (NBMI) to reduce the lung inflammatory response was investigated in mice with a cigarette smoke-induced airway inflammation. Female BALB/c mice were exposed to both sidestream and main-stream cigarette smoke via an inhalation tower once a day (12 cigarettes during 30 min) and a daily dose of NBMI (from 5 to 150 mg/kg) was administered. On day 15 the mice were exsanguinated and the effect on general health status, inflammatory cells and inflammatory markers was investigated. There were minor beneficial effects of NBMI, however, 14 days of cigarettes smoking was not sufficient to induce an inflammatory response.